11. Ray Charles - Night Time Is the Right Time (03:30)
12. Freddie King - Ain't Nobody's Business What We Do (03:39)
13. Della Reese - The Story of the Blues (01:27)
14. Little Milton - Sundown (06:39)
15. The Jimmy Rodgers All Stars - Blues All Day Long (04:46)
16. Esther Phillips - Confessin' the Blues (03:02)
17. Johnny Winter - Third Degree (06:36)
18. Koko Taylor - Wang Dang Doodle (04:54)
19. Joe Turner - Trouble In Mind (02:22)
20. The Paul Butterfield Blues Band - Spoonful (03:21)
21. John Hammond - Smokestack Lightning (02:49)
22. T-bone Walker - How Long Blues (05:17)
23. Canned Heat - Shake, Rattle and Roll (02:32)
24. Carla Thomas - Red Rooster (04:01)
25. Johnnie Taylor - Blues In the Night (03:21)
26. Champion Jack Dupree - Evil Woman (04:22)
27. Paul Butterfield's Better Days - Nobody's Fault but Mine (03:38)
28. Tony Joe White - Polk Salad Annie (03:44)
29. Big Fat Snake - Mercury Blues (02:33)
30. Wilson Pickett - Mustang Sally (03:08)
31. Dr. John - Mess Around (03:13)
32. The Band - Mannish Boy (Live) [feat. Muddy Waters] (feat. Muddy Waters) (06:41)
33. Lorraine Ellison - Try (Just a Little Bit Harder) (03:04)
34. Aretha Franklin - The Thrill Is Gone (From Yesterday's Kiss) (04:40)
35. The Blues Brothers - She Caught the Katy (04:10)
36. John Baldry - I'm Ready (04:14)
37. Emmy Rossum - Nobody Knows You When You're Down and Out (03:19)
38. Freddie & The Dreamers - Kansas City (02:25)
39. Sam & Dave - I Thank You (02:57)
40. Ben E. King - Fever (02:58)
41. Rufus Thomas - Boom Boom (02:48)
42. Albert Collins - Ice Pick (03:09)
43. Foghat - I Just Want to Make Love to You (04:22)
44. Linkin' Louisiana Peps - I Got My Mojo Working (02:49)
45. The Shadows of Knight - Hoochie Coochie Man (03:56)
46. Delta Cross Band - Dust My Broom (03:30)
47. Taj Mahal - Deed I Do (05:08)
48. Brook Benton - Born Under a Bad Sign (03:08)
49. The Best - Back Door Man (03:15)
50. Jack Champion Dupree - Baby, Please Don't Go (02:28)